A RAPID METHOD FOR THE DETERMINATION OF CITRIC ACID

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A rapid method for the determination of citric acid.

Numerous methods, enzymatic and chemical, have been reported for the determination of small amounts of citric acid in biological fluids. Of the chemical methods the majority involve the conversion of the citric acid to pentabromoacetone, which may be estimated by various gravimetric, titrimetric, or calorimetric procedures. The more recent modifications (l-3) of the met.hod have simplified it a...

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A microcolorimetric method for the determination of citric acid.

In 1947 we (1) described a microcolorimetric method, with a range of sensitivity from 10 to 60 y, for the determination of citric acid in small samples of blood and urine. This method was based on the conversion of citric acid to pentabromoacetone and on the subsequent reaction between pentabromoacetone and alcoholic sodium iodide with the development of a yellow color complex. Hydrazine sulfat...

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Citric Acid Determination

Three types of methods have been proposed for the determination of citric acid in urine. Salant and Wise (1) used the unspecific mercuric sulfate reagent of Deniges (2) in a study of the toxicity of citrates. Thunberg (3) developed an enzymatic method employing a dehydrogenase said to be specific for citrate. The remaining methods depend on the so called pentabromoacetone method of Stahre (4), ...

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A simplified photometric method for the determination of citric acid in biological fluids.

Studies on the oxidation and bromination of citric acid to pentabromoacetone were reported as early as 1847 by Cahours (1). This reaction has since formed bhe basis for many of the procedures for determining citric acid. A qualitative test by Stahre (2), reported in 1897, was adapted fol quantitative analysis by Kunz (3). Pucher, Sherman, and Vickery (4) described the first microphotometric met...

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A Rabid Method for the Determination of Citric Acid* by Murray Saffran

Numerous methods, enzymatic and chemical, have been reported for the determination of small amounts of citric acid in biological fluids. Of the chemical methods the majority involve the conversion of the citric acid to pentabromoacetone, which may be estimated by various gravimetric, titrimetric, or calorimetric procedures. The more recent modifications (l-3) of the met.hod have simplified it a...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1948

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)57202-1